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anti nephrin  (Progen Biotechnik)

 
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    Progen Biotechnik anti nephrin
    Anti Nephrin, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+nephrin/anti+nephrin/bio_rxiv__64898__2026__05__11__724358-284-23-25
    Average 86 stars, based on 1 article reviews
    anti nephrin - by Bioz Stars, 2026-09
    86/100 stars

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    other:

    Article Title: Quantitative phenotyping of Nphs1 knockout mice as a prerequisite for gene replacement studies.
    Article Snippet: Steroid-resistant nephrotic syndrome (SRNS) is the second most frequent cause of chronic kidney disease before the age of 25 yr. Nephrin, encoded by NPHS1, localizes to the slit diaphragm of glomerular podocytes and is the predominant structural component of the glomerular filtration barrier.. Biallelic variants in NPHS1 can cause congenital nephrotic syndrome of the Finnish type, for which, to date, no causative therapy is available.. Recently, adeno-associated virus (AAV) vectors targeting the glomerular podocyte have been assessed as a means for gene replacement therapy.

    Incubation:

    Article Title: Pyridoxine supplementation confers protection against SGPL1 R222Q variant sphingosine phosphate lyase insufficiency syndrome
    Article Snippet: To reduce nonspecific binding, sections were blocked with goat serum (1:20, Sigma-Aldrich, Cat# G9023). .. For tissue permeabilization, samples were incubated with 0.1% Triton X-100 for 10 min. Further samples were incubated for a multicolor staining with primary anti-nephrin (1:50, Progen, Cat#: GP-N2) and STED-compatible secondary Alexa Fluor 488 (1:500, Invitrogen, Cat# A-11073) antibodies. .. Samples were mounted in Prolong Diamond media (Invitrogen, Cat#: P36961) and covered with #1.5 glass coverslips.

    Article Title: Aberrantly glycosylated IgG elicits pathogenic signaling in podocytes and signifies lupus nephritis
    Article Snippet: After centrifugation (16,400 g ; 30 minutes; 4°C), supernatants were collected and an identical amount of protein from each lysate was separated on NuPAGE 4%–12% Bis-Tris Gel (Thermo Fisher Scientific). .. Proteins were transferred to a nitrocellulose membrane, which was subsequently blocked for 1 hour using 5% nonfat dry milk or 3% BSA in TBS-T and incubated at 4°C overnight with mouse anti–human CaMK4 (catalog 610276/clone 26, BD Biosciences), anti-NF-κB (p65) (D14E12, Cell Signaling Technology [CST]), anti-nephrin (GP-N2, Progen), mouse anti–human SNAIL (L70G2, CST), and mouse anti–human GAPDH (catalog 649202/FF26A/F9, BD Biosciences). .. The membrane was washed with TBS-T and incubated with anti–rabbit or anti–mouse IgG coupled with HRP (catalogs sc-2004, sc-2020, sc-2005, sc-2473, respectively; Santa Cruz Biotechnology Inc.).

    Staining:

    Article Title: Pyridoxine supplementation confers protection against SGPL1 R222Q variant sphingosine phosphate lyase insufficiency syndrome
    Article Snippet: To reduce nonspecific binding, sections were blocked with goat serum (1:20, Sigma-Aldrich, Cat# G9023). .. For tissue permeabilization, samples were incubated with 0.1% Triton X-100 for 10 min. Further samples were incubated for a multicolor staining with primary anti-nephrin (1:50, Progen, Cat#: GP-N2) and STED-compatible secondary Alexa Fluor 488 (1:500, Invitrogen, Cat# A-11073) antibodies. .. Samples were mounted in Prolong Diamond media (Invitrogen, Cat#: P36961) and covered with #1.5 glass coverslips.

    Immunofluorescence:

    Article Title: Glomage : A multimodal platform for high-content morphological and RNA profiling of glomeruli in zebrafish and mouse models
    Article Snippet: .. The following antibodies and concentrations were used for the immunofluorescence on mouse glomeruli: Anti-Nephrin 1:1000 (Progen, Heidelberg, Germany, Cat. No.: GP-N2), Anti-Dach1 1:500 (Sigma-Aldrich, Cat. No.: HPA012672). .. The same Alexa Fluor 647-conjugated donkey anti-rabbit (Thermo Fisher Scientific) and a Cy3-conjugated donkey-anti guinea pig (Jackson ImmunoResearch, Newmarket, United Kingdom) were used as secondary antibodies.

    Membrane:

    Article Title: Aberrantly glycosylated IgG elicits pathogenic signaling in podocytes and signifies lupus nephritis
    Article Snippet: After centrifugation (16,400 g ; 30 minutes; 4°C), supernatants were collected and an identical amount of protein from each lysate was separated on NuPAGE 4%–12% Bis-Tris Gel (Thermo Fisher Scientific). .. Proteins were transferred to a nitrocellulose membrane, which was subsequently blocked for 1 hour using 5% nonfat dry milk or 3% BSA in TBS-T and incubated at 4°C overnight with mouse anti–human CaMK4 (catalog 610276/clone 26, BD Biosciences), anti-NF-κB (p65) (D14E12, Cell Signaling Technology [CST]), anti-nephrin (GP-N2, Progen), mouse anti–human SNAIL (L70G2, CST), and mouse anti–human GAPDH (catalog 649202/FF26A/F9, BD Biosciences). .. The membrane was washed with TBS-T and incubated with anti–rabbit or anti–mouse IgG coupled with HRP (catalogs sc-2004, sc-2020, sc-2005, sc-2473, respectively; Santa Cruz Biotechnology Inc.).



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    (a) OCT frozen kidney biopsies were sectioned, cleared <t>and</t> <t>immunolabelled</t> followed by confocal imaging and deep-learning segmentation. (b) Maximum intensity projection confocal image of <t>nephrin</t> visualizing the slit diaphragm network and foot processes. (c) Output of the semantic segmentation of both the slit diaphragm and foot processes. Foot processes are marked in red and the slit diaphragm in green. (d) Output of the instance segmentation resulting in segmentation of each individual foot process in the image. Each foot process is presented in an individual color. (e-g) From the 2 segmentation outputs, the morphometric parameters SDL (e), FP circularity (f), FP area and FP perimeter (g) are extracted. (e) Schematic image of how SDL is measured. The length of the slit (in blue) is divided by the surface area (outlined in white) resulting in a length-density measurement of slit coverage. (f) Schematic image of how FP circularity is measured. From the segmentation of the individual foot processes, the FP circularity is calculated using the formula, circularity = 4 π (area/perimeter−2). The result is a value between 0 and 1 where a perfect circle has a circularity of 1 and a very elongated rectangle approaches 0. (g) Foot process size is calculated both by FP area and by FP perimeter. The FP area is the pixel area of each segmented foot process converted to µm 2 and the FP perimeter is the outline of the foot process with it closed at the base.
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    (a) OCT frozen kidney biopsies were sectioned, cleared <t>and</t> <t>immunolabelled</t> followed by confocal imaging and deep-learning segmentation. (b) Maximum intensity projection confocal image of <t>nephrin</t> visualizing the slit diaphragm network and foot processes. (c) Output of the semantic segmentation of both the slit diaphragm and foot processes. Foot processes are marked in red and the slit diaphragm in green. (d) Output of the instance segmentation resulting in segmentation of each individual foot process in the image. Each foot process is presented in an individual color. (e-g) From the 2 segmentation outputs, the morphometric parameters SDL (e), FP circularity (f), FP area and FP perimeter (g) are extracted. (e) Schematic image of how SDL is measured. The length of the slit (in blue) is divided by the surface area (outlined in white) resulting in a length-density measurement of slit coverage. (f) Schematic image of how FP circularity is measured. From the segmentation of the individual foot processes, the FP circularity is calculated using the formula, circularity = 4 π (area/perimeter−2). The result is a value between 0 and 1 where a perfect circle has a circularity of 1 and a very elongated rectangle approaches 0. (g) Foot process size is calculated both by FP area and by FP perimeter. The FP area is the pixel area of each segmented foot process converted to µm 2 and the FP perimeter is the outline of the foot process with it closed at the base.
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    (a) OCT frozen kidney biopsies were sectioned, cleared <t>and</t> <t>immunolabelled</t> followed by confocal imaging and deep-learning segmentation. (b) Maximum intensity projection confocal image of <t>nephrin</t> visualizing the slit diaphragm network and foot processes. (c) Output of the semantic segmentation of both the slit diaphragm and foot processes. Foot processes are marked in red and the slit diaphragm in green. (d) Output of the instance segmentation resulting in segmentation of each individual foot process in the image. Each foot process is presented in an individual color. (e-g) From the 2 segmentation outputs, the morphometric parameters SDL (e), FP circularity (f), FP area and FP perimeter (g) are extracted. (e) Schematic image of how SDL is measured. The length of the slit (in blue) is divided by the surface area (outlined in white) resulting in a length-density measurement of slit coverage. (f) Schematic image of how FP circularity is measured. From the segmentation of the individual foot processes, the FP circularity is calculated using the formula, circularity = 4 π (area/perimeter−2). The result is a value between 0 and 1 where a perfect circle has a circularity of 1 and a very elongated rectangle approaches 0. (g) Foot process size is calculated both by FP area and by FP perimeter. The FP area is the pixel area of each segmented foot process converted to µm 2 and the FP perimeter is the outline of the foot process with it closed at the base.
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    (a) OCT frozen kidney biopsies were sectioned, cleared <t>and</t> <t>immunolabelled</t> followed by confocal imaging and deep-learning segmentation. (b) Maximum intensity projection confocal image of <t>nephrin</t> visualizing the slit diaphragm network and foot processes. (c) Output of the semantic segmentation of both the slit diaphragm and foot processes. Foot processes are marked in red and the slit diaphragm in green. (d) Output of the instance segmentation resulting in segmentation of each individual foot process in the image. Each foot process is presented in an individual color. (e-g) From the 2 segmentation outputs, the morphometric parameters SDL (e), FP circularity (f), FP area and FP perimeter (g) are extracted. (e) Schematic image of how SDL is measured. The length of the slit (in blue) is divided by the surface area (outlined in white) resulting in a length-density measurement of slit coverage. (f) Schematic image of how FP circularity is measured. From the segmentation of the individual foot processes, the FP circularity is calculated using the formula, circularity = 4 π (area/perimeter−2). The result is a value between 0 and 1 where a perfect circle has a circularity of 1 and a very elongated rectangle approaches 0. (g) Foot process size is calculated both by FP area and by FP perimeter. The FP area is the pixel area of each segmented foot process converted to µm 2 and the FP perimeter is the outline of the foot process with it closed at the base.
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    (a) OCT frozen kidney biopsies were sectioned, cleared <t>and</t> <t>immunolabelled</t> followed by confocal imaging and deep-learning segmentation. (b) Maximum intensity projection confocal image of <t>nephrin</t> visualizing the slit diaphragm network and foot processes. (c) Output of the semantic segmentation of both the slit diaphragm and foot processes. Foot processes are marked in red and the slit diaphragm in green. (d) Output of the instance segmentation resulting in segmentation of each individual foot process in the image. Each foot process is presented in an individual color. (e-g) From the 2 segmentation outputs, the morphometric parameters SDL (e), FP circularity (f), FP area and FP perimeter (g) are extracted. (e) Schematic image of how SDL is measured. The length of the slit (in blue) is divided by the surface area (outlined in white) resulting in a length-density measurement of slit coverage. (f) Schematic image of how FP circularity is measured. From the segmentation of the individual foot processes, the FP circularity is calculated using the formula, circularity = 4 π (area/perimeter−2). The result is a value between 0 and 1 where a perfect circle has a circularity of 1 and a very elongated rectangle approaches 0. (g) Foot process size is calculated both by FP area and by FP perimeter. The FP area is the pixel area of each segmented foot process converted to µm 2 and the FP perimeter is the outline of the foot process with it closed at the base.
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    Image Search Results


    (a) OCT frozen kidney biopsies were sectioned, cleared and immunolabelled followed by confocal imaging and deep-learning segmentation. (b) Maximum intensity projection confocal image of nephrin visualizing the slit diaphragm network and foot processes. (c) Output of the semantic segmentation of both the slit diaphragm and foot processes. Foot processes are marked in red and the slit diaphragm in green. (d) Output of the instance segmentation resulting in segmentation of each individual foot process in the image. Each foot process is presented in an individual color. (e-g) From the 2 segmentation outputs, the morphometric parameters SDL (e), FP circularity (f), FP area and FP perimeter (g) are extracted. (e) Schematic image of how SDL is measured. The length of the slit (in blue) is divided by the surface area (outlined in white) resulting in a length-density measurement of slit coverage. (f) Schematic image of how FP circularity is measured. From the segmentation of the individual foot processes, the FP circularity is calculated using the formula, circularity = 4 π (area/perimeter−2). The result is a value between 0 and 1 where a perfect circle has a circularity of 1 and a very elongated rectangle approaches 0. (g) Foot process size is calculated both by FP area and by FP perimeter. The FP area is the pixel area of each segmented foot process converted to µm 2 and the FP perimeter is the outline of the foot process with it closed at the base.

    Journal: medRxiv

    Article Title: Nanoscale Podocyte Morphometrics Predict Disease Progression in IgA Nephropathy

    doi: 10.64898/2026.03.30.26349728

    Figure Lengend Snippet: (a) OCT frozen kidney biopsies were sectioned, cleared and immunolabelled followed by confocal imaging and deep-learning segmentation. (b) Maximum intensity projection confocal image of nephrin visualizing the slit diaphragm network and foot processes. (c) Output of the semantic segmentation of both the slit diaphragm and foot processes. Foot processes are marked in red and the slit diaphragm in green. (d) Output of the instance segmentation resulting in segmentation of each individual foot process in the image. Each foot process is presented in an individual color. (e-g) From the 2 segmentation outputs, the morphometric parameters SDL (e), FP circularity (f), FP area and FP perimeter (g) are extracted. (e) Schematic image of how SDL is measured. The length of the slit (in blue) is divided by the surface area (outlined in white) resulting in a length-density measurement of slit coverage. (f) Schematic image of how FP circularity is measured. From the segmentation of the individual foot processes, the FP circularity is calculated using the formula, circularity = 4 π (area/perimeter−2). The result is a value between 0 and 1 where a perfect circle has a circularity of 1 and a very elongated rectangle approaches 0. (g) Foot process size is calculated both by FP area and by FP perimeter. The FP area is the pixel area of each segmented foot process converted to µm 2 and the FP perimeter is the outline of the foot process with it closed at the base.

    Article Snippet: OCT-frozen biopsy specimens were fixed in PFA, cleared in 4% SDS w/ boric acid and fluorescently immunolabelled for nephrin (R&D Systems, cat. no. AF4269) to visualize the slit diaphragm and podocyte foot processes (FPs) based on the protocol described by Unnersjö-Jess et al (details available in Supplement Methods).

    Techniques: Imaging